alu POLYMERS chain-reaction lab
In this lab we analyzed our DNA and used a thermo-cycler to determine our class of ALU DNA; Heterozygous,or Homogenus.
Scientific Terms
DNA - Deoxyribonucleic acid, a self-replicating material present in all living organisms as the main part of chromosomes
Thermocycler - A machine used to amplify segments of DNA with the Polymerase Chain Reaction (PCR)
Chromosomes - A threadlike structure of acids and protein found in the nucleus of most living cells, carrying genetic information. Helix - structural strands that hold Nucleotides and DNA together
Nuclotide - Basic foundation for the Helix strands and DNA. (Makes up DNA and Helix)
Amino Acids - A (adenine), C (cytosine), G (guanine), T (thymine), most basic building blocks of life.
Acid - Substance that ionizes into Hydrogen ions (H+)
Bases - Substance that ionizes into Hydroxide ions (OH-)
Genes - Are made up of DNA, act as instructions to make molecules called proteins. These blueprints are carried by chromosomes
Protein - Organic compounds that consist of large molecules composed of one or more long chains of amino acids.
DNA - Deoxyribonucleic acid, a self-replicating material present in all living organisms as the main part of chromosomes
Thermocycler - A machine used to amplify segments of DNA with the Polymerase Chain Reaction (PCR)
Chromosomes - A threadlike structure of acids and protein found in the nucleus of most living cells, carrying genetic information. Helix - structural strands that hold Nucleotides and DNA together
Nuclotide - Basic foundation for the Helix strands and DNA. (Makes up DNA and Helix)
Amino Acids - A (adenine), C (cytosine), G (guanine), T (thymine), most basic building blocks of life.
Acid - Substance that ionizes into Hydrogen ions (H+)
Bases - Substance that ionizes into Hydroxide ions (OH-)
Genes - Are made up of DNA, act as instructions to make molecules called proteins. These blueprints are carried by chromosomes
Protein - Organic compounds that consist of large molecules composed of one or more long chains of amino acids.
Materials
- Cheek cells
- Chelex
- Master mix
- Bleach
- Primer mix
- TBE buffer
- Agrose gel
- DNA stain solution
Procedure
I extracted cheek cells from my gums (via saline solution), and extracted DNA from the cluster of cells. After spinning my cell into a cell pellet, I heated my sample, mixed it with Kill-ix. After the heating process, using the thermo-cycler, I let it chill over night. Then I heated it back up to a Luke-warm temperature. After adding luminescent chemical dye into the sample, i ran it through with electricity. Using the control as my guide. (control is to the far right, mine is second from the right) My pattern in the image means that I am a (-/-) witch is the pattern for a homozygous.
Conclusion/Results
We had to do the lab many times but I still enjoyed the process and I think I learned a lot about using pipettes. I did not particularly enjoy putting salt water in my mouth, but the rest of the lab was fun. It turned out that I was a homozygous (-/-) meaning that I have the same alu repeats.
ANALYSIS
Adding luminescent chemical dye into the sample of dna Using the control as my guide. (control is to the far right, mine is second from the right) My pattern in the image means that I am a (-/-) witch is the pattern for a homozygous.